Evaluation of Anti-Oral Pathogen Activity and Safety of Lacticaseibacillus paracasei TISTR 2688, Isolated from Fermented Termite Comb.

Abstract

Objective: This study aimed to evaluate the potential of Lacticaseibacillus paracasei TISTR 2688 to be used in the field of oral health care. Materials and methods: The strain TISTR 2688 was assessed for its antimicrobial activity using agar well diffusion assay, ability to suppress plaque formation on prosthetic teeth, tolerance to lysozyme, antibiotic susceptibility and acute oral toxicity based on Organization for Economic Co-operation and Development (OECD) Guidelines. Results: Cell-free supernatant of the strain TISTR 2688 possessed inhibitory activity against Actinomyces vericosus, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum subsp. polymorphum, Porphyromonas gingivalis, Prevotella intermedia, Streptococcus mutans, S. sanguinis, and S. sobrinus, but exhibited no antifungal activity against Candida albicans. In terms of safety, TISTR 2688 showed the phenotypic antibiotic susceptibility to ampicillin, chloramphenicol, clindamycin, erythromycin, gentamycin, kanamycin, streptomycin and tetracycline. The acute oral toxicity assay in rats with TISTR 2688 at 1 × 1010 CFU/kg body weight demonstrated no mortality results. In addition, no toxicity or evidence of gross pathological alterations was observed. Conclusion: Due to its antibacterial activity against some oral pathogens and safety in term of antibiotic susceptibility test together with no acute oral toxicity, L. paracasei TISTR 2688 tend to have potential for development as an oral health care product. > ว.ทันต.มศว ปีที่ 16 ฉบับที่ 1 พ.ศ. 2566 หน้า 78-88. > SWU Dent J. 2023;16(1): 78-88.