Srinakharinwirot University Dental Journal (E-ISSN 2774-0811)

Survival After 3-Year of Partial Pulpotomy Using Bioactive Cements as Pulp Capping Materials in Adult’s Permanent Teeth with Carious Pulp Exposure

Thanwarat Thonthanan, Warit Powcharoen, Dujrudee Chinwong, Patchanee Chuveera — Mon, 24 Feb 2025 00:00:00 +0000

Objective: The present study aimed to explore the survival rate after 3 years of partial pulpotomy in adult permanent teeth with carious pulp exposure, using ProRoot MTA® and Biodentine™.

Materials and Methods: This study is a follow-up to a previously reported 1-year outcome of a randomized clinical trial investigating the non-inferiority of Biodentine compared to MTA for partial pulpotomy in adults’ permanent teeth with carious pulp exposure (Thai Clinical Trials Registry: TCTR20171228003). All 58 participants whose teeth were treated for at least 3 years were contacted for follow-up, which included clinical examinations, radiographic evaluations, and a patient satisfaction survey.

Results: Forty-eight patients (82.8% recall rate) were followed up for an average of 40.95 ± 4.24 months. The overall cumulative pulp survival rate was 78.7%, with 87.4% for ProRoot MTA® and 70.7% for Biodentine™, showing no significant difference between the materials. The highest frequency of failure occurred between 24 and 35 months. Discoloration of teeth was observed in both groups, 42.1% (8/19) in teeth treated with ProRoot MTA® and 10% (2/20) in teeth treated with Biodentine™, showing a statistically significant difference (p = 0.031). Acceptable restorations were present in 74.4% of patients, and most patients were very satisfied with the treatment.

Conclusion: The cumulative pulp survival rate for adult permanent teeth with carious pulp exposure was 78.7% after at least 3 years of partial pulpotomy, with no significant difference between ProRoot MTA® and Biodentine™. Since treatment failures were most frequent between 24 and 35 months, clinical and radiographic follow-ups are recommended for at least 3 years. For patients with significant tooth structure loss who have not received full coverage restoration, annual follow-ups are advised to assess restoration quality.

The Study of Satisfaction on Chewing Jelly with Coconut Oil for Oral Moisturization in the Elderly: A Pilot study

Panurak Kaewnoi, Serena S. Sakoolnamarka, Sorasun Rungsiyanont — Mon, 24 Feb 2025 00:00:00 +0000

Dry mouth problems are common among the elderly, and many products have been developed to relieve and treat this issue.

Objectives: This research aimed to develop clinical innovations for saliva stimulation and oral moisturizing in the elderly using chewing jelly containing coconut oil. A pilot study of its satisfaction was performed.

Materials and Methods: The study population was selected based on pre-defined inclusion criteria from elderly members of the Nong Sadao Elderly Club, Suphan-Buri. Participants were aged 60 years and above and self-reported dry mouth conditions. Data were collected through interviews using satisfaction questionnaires that addressed appearance, mouthfeel, and oral moisturization after chewing two jelly formulas containing coconut oil. Descriptive statistics summarized basic data, such as mean and standard deviation. Differences in satisfaction across demographics were tested using the Mann-Whitney U Test, and differences between the original and herbal formula jellies were tested using the Wilcoxon Signed-Rank Test, with a confidence level of 95%.

Results: The study population consisted of 30 elderly individuals with an average age of 70.20 ± 3.70 years. Participants included 6 males (20%) and 24 females (80%). Most participants were satisfied with the innovation, rating the appearance of both formula jellies the highest, followed by oral moisture. The lowest score was for the mouthfeel. The group experiencing moderate to severe dry mouth rated the overall appeal of the herbal formulation significantly higher. The overall scores for appearance and moisturization satisfaction indicated a statistically significant difference, with the herbal formula scoring higher.

Conclusion: The chewing jelly showed high overall satisfaction. Overall, the herbal formula received higher score than the original formula.

Human Osteoclasts Enhance Osteogenic Differentiation of Bone Stromal Cells from Mandibular Tori

Mon, 24 Feb 2025 00:00:00 +0000

Objective: To determine the effect of human osteoclasts on osteogenic differentiation of bone stromal cells via the receptor activator of nuclear factor kappa B (RANK)-RANK ligand (RANKL) reverse signaling.

Materials and Methods: Human peripheral blood mononuclear cells were cultured with stimulating factors until they became multinucleated mature osteoclasts. After being identified for the characteristics of mature osteoclasts, their conditioned medium (OC-CM) was collected. Bone stromal cells harvested from mandibular tori of four patients were treated with OC-CM prior to assessments of osteogenic gene expressions, differentiation, and biomineralization. Both the osteoprotegerin (OPG)-pretreated bone stromal cells and the conditioned medium from GW4869-treated mature osteoclasts (GW-OC-CM) were analyzed for suppression of osteogenic induction in order to investigate the inducible effect of OC-CM.

Results: The OC-CM significantly upregulated expressions of osteogenic genes and enhanced differentiation and biomineralization of bone stromal cells (p < 0.05). Pretreatment with OPG, a decoy receptor of RANKL, significantly reduced the inducible effects of OC-CM (p < 0.05). Similarly, the upregulated expressions and enhanced biomineralization were also significantly diminished bytreatment with GW-OC-CM (p < 0.05).

Conclusion: Mature osteoclasts can induce osteogenic differentiation of bone stromal cells possibly via the RANK-RANKL reverse signaling.

Effect of Saliva Storage Conditions on Bacterial DNA Quantification by Real-time Polymerase Chain Reaction

Mon, 24 Feb 2025 00:00:00 +0000

Objective: This research aimed to study the effects of temperature and duration of saliva samples storage on quantification of bacterial DNA by Real-time polymerase chain reaction (Realtime PCR)

Materials and Methods: Human saliva samples were collected from 3 healthy volunteers, aliquoted into microcentrifuge tubes and stored in the following conditions; centrifuged immediately before storing at -80^oC, kept at room temperature, on ice, or dry ice for 6 hours, 24 hours or 120 hours. Genomic DNA was extracted for quantitative analysis of total bacteria by Real-time PCR.

Results: Absolute quantitative real-time PCR showed that concentrations of bacterial DNA from saliva samples stored at almost every condition which were stored at room temperature for 120 hours, on ice for every duration, or on dry ice for 6 or 24 hours were similar to that of the control, saliva centrifuged and frozen immediately after collection. However, bacterial DNA concentration from saliva stored at room temperature for 6 or 24 hours was significantly higher than that of the control. In contrast, the concentration of DNA from saliva stored on dry ice for 120 hours was significantly lower than that of the control.

Conclusion: Temperature and duration had effects on bacterial DNA quantity. Saliva storage at room temperature within 24 hours had increasing bacterial DNA as quantify by Real-time PCR. Saliva storage on ice and dry ice could maintain bacterial DNA similar to sample centrifugation immediately and then frozen at -80^oC. But long duration of storage, 120 hours, would decrease bacterial quantity.

Effect of Building Orientation on Marginal Gap and Internal Fit of The Implant-Supported 3D-Printed Provisional Crown

Mon, 24 Feb 2025 00:00:00 +0000

Background: With the increasing popularity of immediate implant placement and restoration, precise fabrication methods for provisional restorations have become crucial since it helps preserving the soft tissue around the implant. 3D-printed PMMA provides cost-effective alternatives despite concerns about polymerization shrinkage. Previous studies have shown that building orientation for 3D-printed methods can affect the marginal gap and internal fit of the restoration. This study aims to examine the effect of building orientation on the marginal gap and internal fit of implant-supported provisional prostheses.

Materials and Methods: The implant-supported provisional restoration of the right maxillary central incisor was designed by complete digital workflow using 3shape software. The virtual implant crowns were fabricated with 3D-printer (DLP technology) in three different building orientations (0°, 45°, and 90°) with 10 samples per group. The samples underwent post-processing methods according to the manufacturer’s recommendation. Marginal gap and internal fit examination were conducted using a digital silicone replica technique and superimposition method. After digitizing the silicone replica into .stl file, the gap distances were measured at sixteen reference points for each sample using Artec Studio 18 software. The degree of discrepancy was reported in color mapping. The measurements were statistically analyzed using One-way ANOVA .

Results: Statistical analysis revealed significant differences in marginal, cervical, and axial gap across the three groups (p < 0.05) except the occlusal gap. The 45° group had a significantly smaller marginal gap (19.1 ± 6.98 μm) than the 0° group (29.78 ± 10.63 μm) and the 90° group (35.68 ± 18.37 μm). Color mapping indicated thicker cement space around the margin of the Ti-base abutment in the 0° and 90° groups compared to the 45° group.

Conclusion: Building orientation in 3D printing significantly affects the marginal gap and internal fit of implant-supported provisional restorations. The 45° orientation produced the best results among the three groups.

Knowledge and Attitudes Towards Emergency Management of Traumatic Dental Injuries Among Elementary School Teachers in Sakaeo, Thailand

Kanchana Hemantakul, Praewpat Pachimsawat, Pornpoj Fuangtharnthip — Mon, 24 Feb 2025 00:00:00 +0000

Objective: This study aimed to evaluate the knowledge and attitudes of elementary school teachers towards the emergency management of traumatic dental injuries (TDIs) and to identify their associated factors.

Materials and Methods: A cross-sectional study was conducted using a structured selfadministered questionnaire distributed to 385 elementary school teachers across 31 schools in Sakaeo province. The questionnaire assessed demographic characteristics, knowledge, and attitudes regarding the emergency management of TDIs. A validity test evaluated the questionnaire through content validity, and a reliability test through Cronbach’s alpha. Knowledge was evaluated through two scenario cases involving fractured teeth and avulsion. Descriptive analysis and Pearson Chi-square tests were employed to analyze the data and examine the association between knowledge and teachers’ characteristics at a significance level of 0.05.

Results: The study revealed significant gaps in knowledge, particularly regarding the evaluation period for neurological symptoms (14.0% correct responses) and ideal replantation time (31.9%). Conversely, a majority of teachers correctly answered the questions on permanent tooth identification (74.5%) and on-site management of fractured teeth (70.9%). Regarding attitudes, a substantial proportion of teachers (86.0%) acknowledged their responsibility to assist students in dental emergencies, and most recognized the importance of permanent teeth for a high quality of life (96.6%). However, only 22.3% of teachers expressed confidence in managing TDIs. No association of overall knowledge was found with teachers’ characteristics.

Conclusions: Our study indicates that while elementary school teachers in Sakaeo province have insufficient knowledge about managing TDIs, their attitudes towards handling such emergencies are generally positive. This suggests a need for targeted training programs to enhance teachers’ knowledge and confidence in managing TDIs effectively.

The Remineralization Effect of Theobromine and CPP-ACP on Enamel of Primary Molars

Krairavee Theeranut, Varisara Sirimaharaj, Sithichai Wanachantarak — Mon, 24 Feb 2025 00:00:00 +0000

Background: Studies have investigated remineralization process utilizing non-fluoridated agents, however the efficacy of theobromine and casein phosphopeptide-amorphous calcium phosphate (CPP ACP) remain relatively scarce, especially in primary teeth. Objective: This study aimed to investigate the remineralization effects of theobromine and CPP-ACP on enamel of primary molars under carious simulation condition.

Methods: Extracted forty-five primary molars were collected. The crown was divided into 4 pieces. Three samples from each crown were randomly assigned to one of three treatment groups: deionized water (control), theobromine, and CPP-ACP for microhardness test and another sample was randomly used for ultrastructure and Energy-Dispersive X-ray Spectroscopy (EDS) evaluation. Microhardness testing was applied before and after pH cycling for all three groups (n = 15 each). Ultrastructure and EDS analyzed were performed on samples from four groups: untreated, deionized water, theobromine, and CPP-ACP (n = 10 each). The 7 days pH cycling process was employed to simulate a caries model. Data were described and compared statistically.

Result: After pH cycling, the microhardness of enamel decreased in all 3 groups. The theobromine group did not exhibit significant changes after pH cycling (220.72 ± 24.75 VHN from 275.84 ± 25.34) compared to deionized water (163.21 ± 28.08 from 275.18 ± 27.71 VHN) and CPP-ACP (184.74 ± 44.55 from 277.29 ± 28.48 VHN) groups. Theobromine and CPP-ACP groups showed mineral precipitation covered demineralized enamel surface, as well as deionized water group. The Wt.% of Ca and P elements in theobromine group (Ca 39.54 ± 4.11 and P 18.38 ± 1.61) were not significantly different from untreated enamel (43.33 ± 5.48 and 19.66 ± 1.70) while CPP-ACP (Ca 36.37 ± 3.77 and P 17.13 ± 1.71) and deionized water groups (Ca 36.88 ± 4.67 and P 17.24 ± 2.10) showed a significant reduction (p < 0.05).

Conclusion: Under the carious simulation conditions, theobromine might induce remineralization on demineralized enamel by increased Ca and P elements concentration and improved the microhardness while CPP-ACP did not show significant change from the deionized water group.

The Accuracy of Single Tooth Mini Dental Implant Placement Using Computer Assisted Surgical Guide: A Randomized Clinical Trial Comparative Study

Voravan Vorasubin, Weerapan Aunmeungthong, Pathawee Khongkhunthian — Mon, 24 Feb 2025 00:00:00 +0000

Background: This study aimed to assess the accuracy of mini dental implant placement using conventional surgical templates (CST) versus digital (computer assisted) surgical drill guides (SDG) for single tooth prosthesis.

Materials and methods: Sixteen participants recruited according to inclusion criteria. Twenty implants (16 participants) were randomly assigned to either the CST or SDG group. Mini dental implants were placed in the lower anterior and premolar area. The positioning of implants were analyzed and compared using Planmeca Romexis™.

Results: Statistical analysis using the Shapiro-Wilk test and independent sample T-test revealed significant differences between the two groups in 3 parameters out of 10 parameters: top horizontal deviation (CST; 1.43 ± 0.77 and SDG; 0.67 ± 0.3 mm), top global deviation (CST; 1.83 ± 0.85 and SDG; 0.82 ± 0.52 mm) and angular deviation (CST; 5.53 ± 3.14 and SDG; 1.36 ± 0.7 degrees) at p < 0.05.

Conclusion: CST and SDG are effective for placing single-tooth mini dental implants in limited-ridge spaces, especially in the anterior and premolar regions. Their versatility allows for adaptation to the specific circumstances of each case, ultimately enhancing clinical decision-making and patient outcomes.

Fabrication and Clinical Evaluation of a Novel 3D printed Hydroxyapatite/Polycaprolactone Composite (Novel 3DP HA/ PCL) for Maxillary Sinus Augmentation: A Preliminary Study

Mon, 24 Feb 2025 00:00:00 +0000

Objective: To fabricate a 3DP hydroxyapatite/polycaprolactone composite graft material and assess its clinical efficacy in maxillary sinus augmentation through a preliminary assessment of bone density changes over a one-year period, compared to deproteinized bovine bone mineral graft.

Methods: A 3DP hydroxyapatite/polycaprolactone (HA/PCL) composite was fabricated using 3D printing of calcium sulfate-based material, followed by phase transformation and PCL infiltration. The composite was characterized through SEM, XRD, micro-CT, and compression testing. In a clinical study, 3DP HA/PCL composite material was compared with deproteinized bovine bone graft in sinus augmentation procedures. Cone-beam computed tomography (CBCT) was used to measure bone density at baseline, 6 months, and 1-year post-operation.

Results: SEM and micro-CT analyses revealed that the 3DP HA/PCL composite exhibited a highly porous, three-dimensional architecture with HA crystals combined with PCL. The microstructure was characterized by a mixture of spherical and irregular-shaped particles with 60.67% porosity. Compression testing demonstrated that the 3DP HA/PCL composite granules had a compressive load resistance of 7.55 ± 1.71 N. The calculated compressive strength of the granule was approximately 2.4 MPa. CBCT analysis of bone density changes revealed distinct patterns between the two groups. Significant differences in graft bone density were observed in the control group at all time points (P < 0.05) , while the 3DP HA/PCL group demonstrated no significant changes (P < 0.3831). However, between 6 months to 1 year, the 3DP HA/PCL group exhibited an increased bone density gain trend similar to the rate observed in the xenograft group. At 1 year, the increase in bone density from T1 to T3 was significant in both the control and test groups., These findings indicate that while 3DP HA/PCL grafts initially increase bone density more slowly than xenografts, they demonstrate a more pronounced gain in the later phase compared to the early phase.

Conclusion: Based on the promising preliminary results from the sinus augmentation study, 3DP HA/PCL composite demonstrates potential as an alternative bone graft material to deproteinized bovine bone mineral.

Editorial Board, SWU Dent J. Volume 18 No.1 (January-June) 2025

วิทยาสารทันตแพทยศาสตร์ มหาวิทยาลัยศรีนครินทรวิโรฒ — Mon, 24 Feb 2025 00:00:00 +0000

สวัสดีท่านผู้อ่านทุกท่านครับ สำหรับวิทยาสารทันตแพทยศาสตร์ มหาวิทยาลัยศรีนครินทรวิโรฒ ฉบับที่ 1 ประจำปี พ.ศ.2568 ทางกองบรรณาธิการฯ ได้คัดสรรผลงานทางวิชาการที่หลากหลายและมีคุณภาพมาเผยแพร่เช่นเดิมครับ สำหรับเล่มนี้ประกอบไปด้วยบทวิทยาการจำนวน 9 บทความ ครอบคลุมสาขาทันตกรรมชุมชนทันตกรรมสำหรับเด็ก ชีววิทยาช่องปาก ทันตกรรมรากเทียม และวิทยาเอนโดดอนด์
ทั้งนี้ช่วงปลายปี พ.ศ. 2567 ที่ผ่านมา คณะทันตแพทยศาสตร์ มหาวิทยาลัยศรีนครินทรวิโรฒ เป็นเจ้าภาพในการจัดงานประชุมขององค์กรผู้บริหารคณะทันตแพทยศาสตร์แห่งประเทศไทย (อ.บ.ท.ท.) ครั้งที่ 21 ระหว่างวันที่13–15 พฤศจิกายน พ.ศ.2567 ณ อำเภออัมพวา จังหวัดสมุทรสงคราม และเปิดโอกาสให้ผลงานทางวิชาการที่ผ่านการคัดเลือกให้นำเสนอในงานประชุมดังกล่าว สามารถเลือกเผยแพร่ผลงานลงเล่ม ว.ทันต.มศว ปีที่ 18 ฉบับที่ 1พ.ศ. 2568 ได้ โดยต้องผ่านกระบวนการคัดเลือกและพิจารณาผลงานทางวิชาการตามมาตรฐานการพิจารณบทความของ SWU Dent J.
ดังนั้นบทความทั้งหมดที่ลงตีพิมพ์ในวารสารฉบับนี้ จึงเป็นบทความซึ่งส่งมาร่วมนำเสนอในงานประชุมดังกล่าวบทความทั้งหมดที่ตีพิมพ์ในเล่มนี้ ผ่านการนำเสนอผลงานในรูปแบบ Oral Presentation หรือ Poster Presentation ในสถานที่จัดงานโดยไม่มีการบันทึกวีดิโอเผยแพร่ มีเพียงบทคัดย่อของบทความเท่านั้นที่ลงเผยแพร่ในเล่มสูจิบัตรและรายงานสืบเนื่องการประชุมวิชาการของงานประชุมฯ
เริ่มตั้งแต่เล่มนี้เป็นต้นไป ทางกองบรรณาธิการได้มีการปรับคณะทำงานเล็กน้อย รวมทั้งเพิ่มเติมกองบรรณาธิการ รุ่นใหม่ไฟแรงมาเสริมการทำงาน พร้อมทั้งมีการปรับการจัดรูปแบบตีพิมพ์ให้เป็นไปตามสากลนิยมมากขึ้น และเรายังคงยึดมั่นพัฒนาคุณภาพวารสารฯ เพื่อเป็นช่องทางในการเผยแพร่ ส่งเสริมสนับสนุนงานวิจัยและวิชาการและเป็นสื่อสัมพันธ์ทางวิชาการในสาขาทันตแพทยศาสตร์และสาขาวิชาการที่เกี่ยวข้อง สำหรับรายละเอียดท่านผู้อ่านสามารถดูข้อมูลได้ตั้งแต่เล่มนี้เป็นต้นไปครับ
ในฐานะตัวแทนของกองบรรณาธิการฯ ผมขอขอบพระคุณผู้นิพนธ์ทุกท่านที่ได้มอบความไว้วางใจให้วิทยาสารทันตแพทยศาสตร์ มหาวิทยาลัยศรีนครินทรวิโรฒ เป็นช่องทางในการเผยแพร่ผลงานทางวิชาการ และผมขอขอบพระคุณผู้ทรงคุณวุฒิทุกท่านที่ได้อุทิศและทุ่มเทแรงกายใจในการพิจารณาบทความทางวิชาการให้ถูกต้องและเป็นไปตามมาตรฐานครับ ซึ่งความพยายามมุ่งมั่นรักษาคุณภาพมาอย่างต่อเนื่อง จึงทำให้ SWU Dent J. ได้รับ การรับรองคุณภาพวารสารวิชาการ “กลุ่มที่ 1 และ ACI” ตามประกาศผลการประเมินคุณภาพวารสารวิชาการที่อยู่ในฐานข้อมูลศูนย์ดัชนีการอ้างอิงวารสารไทย THAI-JOURNAL CITATION INDEX (TCI) รอบที่ 5 (รับรองคุณภาพวารสารเป็นเวลา 5 ปี ระหว่าง พ.ศ. 2568-2572)
และวิทยาสารทันตแพทยศาสตร์ มหาวิทยาลัยศรีนครินทรวิโรฒ ยังคงเปิดรับบทความทางวิชาการประเภทต่าง ๆ ทั้งบทความวิจัย บทความปริทัศน์ และรายงานผู้ป่วยที่น่าสนใจทั้งภาษาไทยและภาษาอังกฤษตลอดทั้งปีครับ จึงขอเชิญชวนผู้นิพนธ์ทุกท่านส่งบทความทางวิชาการด้านทันตแพทยศาสตร์และสาขาที่เกี่ยวข้อง ตามช่องทางที่ได้ แนะนำไว้ทางหน้าเว็บไซต์ออนไลน์ของวิทยาสารฯ สุดท้ายนี้ผมขออำนวยพรให้กองบรรณาธิการทุกท่าน ท่านผู้ทรงคุณวุฒิ ผู้นิพนธ์และผู้อ่านทุกท่าน มีแต่ความสุขกายสุขใจ ปราศจากโรคภัยไข้เจ็บ และคิดสมหวังในทุก ๆ สิ่งตามที่ท่านปรารถนา ตลอดทั้งปีนี้ครับ
สวัสดีปีใหม่ พ.ศ. 2568 อีกครั้งครับ
ผศ.ดร.ทพ.ชัชพันธุ์ อุดมพัฒนากร บรรณาธิการวิทยาสารทันตแพทยศาสตร์ มหาวิทยาลัยศรีนครินทรวิโรฒ