Human Osteoclasts Enhance Osteogenic Differentiation of Bone Stromal Cells from Mandibular Tori

Authors

  • Sumit Suamphan School of Dentistry, Mae Fah Luang University, Chiang Rai 57100, Thailand.
  • Anupong Makeudom School of Dentistry, Mae Fah Luang University, Chiang Rai 57100, Thailand.
  • Ekapong Dechtham School of Dentistry, Mae Fah Luang University, Chiang Rai 57100, Thailand.
  • Piyanat Sangangam School of Dentistry, Mae Fah Luang University, Chiang Rai 57100, Thailand.
  • Suttichai Krisanaprakornkit School of Dentistry, Mae Fah Luang University, Chiang Rai 57100, Thailand.
  • Chidchanok Leethanakul Orthodontic Section, Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Songkhla 90112, Thailand.

Keywords:

Bone Mineralization, Osteoblast, Osteoclast, Osteoprotegerin, RANK Ligand

Abstract

Objective: To determine the effect of human osteoclasts on osteogenic differentiation of bone stromal cells via the receptor activator of nuclear factor kappa B (RANK)-RANK ligand (RANKL) reverse signaling. Materials and Methods: Human peripheral blood mononuclear cells were cultured with stimulating factors until they became multinucleated mature osteoclasts. After being identified for the characteristics of mature osteoclasts, their conditioned medium (OC-CM) was collected. Bone stromal cells harvested from mandibular tori of four patients were treated with OC-CM prior to assessments of osteogenic gene expressions, differentiation, and biomineralization. Both the osteoprotegerin (OPG)-pretreated bone stromal cells and the conditioned medium from GW4869-treated mature osteoclasts (GW-OC-CM) were analyzed for suppression of osteogenic induction in order to investigate the inducible effect of OC-CM. Results: The OC-CM significantly upregulated expressions of osteogenic genes and enhanced differentiation and biomineralization of bone stromal cells (p < 0.05). Pretreatment with OPG, a decoy receptor of RANKL, significantly reduced the inducible effects of OC-CM (p < 0.05). Similarly, the upregulated expressions and enhanced biomineralization were also significantly diminished bytreatment with GW-OC-CM (p < 0.05). Conclusion: Mature osteoclasts can induce osteogenic differentiation of bone stromal cells possibly via the RANK-RANKL reverse signaling.

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Author Biographies

Sumit Suamphan, School of Dentistry, Mae Fah Luang University, Chiang Rai 57100, Thailand.

First Author

Anupong Makeudom, School of Dentistry, Mae Fah Luang University, Chiang Rai 57100, Thailand.

Co-author

Piyanat Sangangam, School of Dentistry, Mae Fah Luang University, Chiang Rai 57100, Thailand.

Co-author

Suttichai Krisanaprakornkit, School of Dentistry, Mae Fah Luang University, Chiang Rai 57100, Thailand.

Co-author

Chidchanok Leethanakul, Orthodontic Section, Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Songkhla 90112, Thailand.

Corresponding author

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Published

2025-02-24

How to Cite

1.
Suamphan S, Makeudom A, Dechtham E, Sangangam P, Krisanaprakornkit S, Leethanakul C. Human Osteoclasts Enhance Osteogenic Differentiation of Bone Stromal Cells from Mandibular Tori. SWU Dent J. [Internet]. 2025 Feb. 24 [cited 2025 Mar. 9];18(1):39-4. Available from: https://ejournals.swu.ac.th/index.php/swudentj/article/view/16654

Issue

Vol. 18 No. 1 (2025): Srinakharinwirot University Dental Journal (Online)

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บทวิทยาการ (Original articles)

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