Association between Bifidobacterium, Fusobacterium nucleatum and Type of Root Canal Infections in Primary Teeth and Clinical Symptom: A Quantitative Real-Time PCR Analysis

Authors

  • Panthita Piyasoonthorn Pediatric dentist, Ranong Hospital, Ranong 85000, Thailand.
  • Kemthong Mitrakul Department of Pediatric Dentistry, Faculty of Dentistry, Mahidol University, 6, Yothi Road, Ratchathewi District, Bangkok 10400, Thailand

Keywords:

Root canal infection, Pulpectomy, Primary teeth, Real-Time PCR, F. nucleatum, Bifidobacterium

Abstract

Objective: To quantify levels of Bifidobacterium and Fusobacterium nucleatum in two typesof primary teeth root canal which are irreversible pulpitis and pulp necrosis and to analyze theassociation between these bacteria, clinical symptoms and radiographic findings. Materials and Methods: Subjects were chosen from patients aged 2-10 years old who cameto the Pediatric Dental Clinic, Faculty of Dentistry, Mahidol University and needed pulpectomy treatmentfor this cross-sectional study. Pulpal diagnosis based on the American Academy of PediatricDentistry. Recorded clinical signs and symptoms including pre-operative radiographs before treatment.Collected fluid inside root canals using paper points by aseptic technique. Performed DNA extractionand quantitative real-time PCR using fluorescent dye (SYBR green) using specific primers to identifiedBifidobacterium and F. nucleatum. Results: Total of 134 primary teeth was selected. Subjects consisted of 70 males (52%) and 64 females (48%). Mean (± standard deviation) age was 5.25 ± 1.37 years old. Sixty eight samples were diagnosed with irreversible pulpitis (51%) and 66 with pulp necrosis (49%). Amounts of total bacteria (p ≤ 0.001), F. nucleatum (p = 0.025) and Bifidobacterium (p = 0.183) in the pulp necrosis group were higher than in the irreversible pulpitis group. The ratio of Bifidobacterium to total bacteria was higher in irreversible pulpitis group (p = 0.016). There was a correlation between levels of F.nucleatum and swelling at gingiva area present clinically. Conclusion: Levels of total bacteria and F. nucleatum were significantly higher in pulpnecrosis group. F. nucleatum was correlated with swelling at gingiva area present clinically.

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Author Biography

Kemthong Mitrakul, Department of Pediatric Dentistry, Faculty of Dentistry, Mahidol University, 6, Yothi Road, Ratchathewi District, Bangkok 10400, Thailand

correspondence

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Published

2023-09-13

How to Cite

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Piyasoonthorn P, Mitrakul K. Association between Bifidobacterium, Fusobacterium nucleatum and Type of Root Canal Infections in Primary Teeth and Clinical Symptom: A Quantitative Real-Time PCR Analysis. SWU Dent J. [Internet]. 2023 Sep. 13 [cited 2024 Dec. 11];16(2):99-113. Available from: https://ejournals.swu.ac.th/index.php/swudentj/article/view/15451

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