Anticariogenic Activity of Garcinone B from Garcinia mangostana

Abstract

AbstractObjectives: The aim of the present study was to evaluate anticariogenic activity of garcinoneB isolated from Garcinia mangostana Linn. pericarp extract.Materials and Methods: Garcinone B was evaluated for their antibacterial activity againstcariogenic bacteria by Kirby-Bauer disk diffusion method. Cariogenic bacteria tested in this studywere Streptococcus mutans, Streptococcus sobrinus, Lactobacillus acidophilus, Lacticaseibacillus caseiand standard strains (S. mutans ATCC 25175). Time-killed assay and hydrophobicitywere also determined.Results: We found that garcinone B was effective for all bacterial tests. It exhibited strongantibacterial activity against cariogenic bacteria with the range of MIC value of 0.25–0.5 μg/ml andzone of inhibition of 6-14 mm. The garcinone B exhibited strong antibacterial activity against mutans Streptococci and Lactobacillus with MIC value of 0.25 μg/ml and 0.5 μg/ml, respectively. In thetime-kill curve, garcinone B was bactericidal against S. mutans ATCC 25175 at the concentration of1 μg/ml, and completely killed the bacteria within 1 h at the concentration of 2 μg/ml. A decreasedcell surface hydrophobicity of S. mutans ATCC 25175 induced by garcinone B was found.Conclusions: These findings reveal that garcinone B has potential for antibacterial activityagainst cariogenic microorganisms. In addition, garcinone B has potential candidate for alternativechemotherapeutic approaches to dental caries.Keyword: Antibacteria, Garcinone B, Lactobacillus, Mangosteen, S. Mutans.SWU Dent J. 2021;14(2):25-34.