The Decrease of Rouleaux Formation of Red Blood Cells in Healthy Human by Water-Soluble Chlorophyll as Revealed by Scanning Electron Microscopy

Abstract

Objective: In present study, we examined rouleaux formation of red blood cells before and after drinking water-soluble chlorophyll as anantioxidant. Method: In blood smear, red blood cells of 20 volunteers were examined before and after drinking water-soluble chlorophyllby using a light microscope, erythrocyte sedimentation rate (ESR) and scanning electron microscopy (SEM). Results: The rouleauxformations were modularly found in 30 and 60 min. In contrast, those formations rarely appeared in 45 min. ESR values of both sexesafter drinking water-soluble chlorophyll for 45 min were significantly lower than those before drinking. In SEM, before drinking watersolublechlorophyll, rouleaux formations were found. After drinking water-soluble chlorophyll for 45 min, however, those formationsdisappeared. Conclusion: The results suggest that water-soluble chlorophyll may be able to decrease rouleaux formations of red bloodcells in healthy volunteers.Keywords: water-soluble chlorophyll, rouleaux formation, red blood cell, scanning electron microscopyThai Pharm Health Sci J 2009;4(4):450-455§