Transfection of Cells in Suspension Using Sonoporation Method

Abstract

Sonoporation has an important role in biomedicine, drug delivery and biotechnology. Development of nonviral method would be a valuable alternative for gene therapy. Sonoporation represents nonviral transfection method. This method has been widely used to transfect genetic materials to living cells. Sonoporation creates opportunities for numerous transfections of cells in suspension. This paper describes a sonoporation method based on ultrasound and also presents relevant studies on the sonoporation demonstrating the use of ultrasound to produce transient pores in cell membrane which allows transfection to occur. The paper discusses the design of the sonoporation system based upon the ultrasonic wave and acoustic cavitation. Finally, the paper also presents applications of sonoporation in gene and cell transfection.