This research aimed to study the opinion on work performance and the developingguidelines on the emergency medical service system (EMSs), compare the knowledge levels on theEMSs of practitioners in Kaengkhro District, Chaiyaphum Province. The samples were 206 practitionersincluding nurse, officers or foundation volunteers, emergency medical officers, driver officers cradleduty officers. The instrument for collecting the data consisted of the checklists the test of knowledgewith the 0.92 reliability value. Frequency, percentage, mean, standard deviation, t-test and One-wayANOVA were applied for data analysis.