Currently, the opportunistic infection from Scedosporium apiospermum has beenincreasing in immunocompromised host, due to this pathogen could be detected in environment. Thus, the study of virulence factors are important for assessment the severity and disease pathogenesis. The aim of this study was to investigate the activity of phospholipase, esterase and hemolysin in S. apiospermum. The activity of phospholipase, esterase and hemolysin performedby simple egg yolk agar, tween 80 opacity test agar and Brain Heart Infusion agar, respectively. The Pz score calculation were performed to analyse the results. This showed the expression of esterase and hemolysin from S. apiospermum. Both of esterase and hemolysin are the virulencefactors, which usually found in Candida albicans and Aspergillus spp. Thus, the study of esterase, hemolysin and phospholipase secretion in S. apiospermum are important for pathogenesis and severity analysis to find out the specific enzyme in the nearly future.
