Science Essence Journal Srinakharinwirot Science Journal en-US (Science Essence Journal) (Science Essence Journal) Fri, 21 Jul 2023 00:00:00 +0000 OJS 60 Investigating Wireless Sensor Kit for Horizontal Circular Motion Analysis <p>Circular motion is a challenging topic in physics education, primarily due to the multiple quantities involved, such as linear and angular quantities, as well as Newton's laws of motion. This often leads to confusion among students. In this study, we developed an experimental device for uniform circular motion using an ESP8266 microcontroller, an IR sensor, an accelerometer, and a gyroscope sensor. The device consists of a microcontroller, sensors, battery, and metal block, which are connected to a circular plate. The device wirelessly transmits data to a preferred display device. We investigated the relationship between centripetal acceleration, angular velocity, and the radius of motion. The results revealed a direct variation between centripetal acceleration and both angular velocity and moving radius, consistent with the centripetal acceleration equation. Additionally, this device is cost-effective and provides students with real-time physical data on circular motion, making it a valuable tool for classroom use, thereby enhancing students' educational experience.</p> Teewin Mahachok, Surawut Wicharn, Suwan Plaipichit Copyright (c) 2023 Science Essence Journal Fri, 21 Jul 2023 00:00:00 +0000 A Potential of Mutant Yeast Strain for Improvement Arabica Coffee Fermentation Process <p><strong>Arabica c</strong>offee is a worldwide popular beverage. Coffee fermentation is important process to enhance the coffee flavor quality. Microorganisms involved in the process are the main factor affecting coffee quality. This study aims to apply the new starter culture of <em>Wickerhamomyces anomalus</em> UV22-3, a UV mutant strain, for coffee fermentation and to improve arabica coffee beverage quality. The results showed that <em>W. anomalus</em> UV22-3 as a starter culture for coffee fermentation could enhance the coffee flavor quality compared to the control experiment (without inoculum). Fermented arabica coffee by strain UV22-3 showed a higher cupping score than wild type and a control condition with unique cupping notes. According to the flavor profile evaluated by Q-graders, the result of this sensory evaluation is 82. Microbial population in the fermentation broth was evaluated. The total yeast number was stable, while the total bacteria was higher after 24 h of strain UV22-3 fermentation. The pH value slightly decreased when the total dissolved solid increased. This research is one alternative to improve the quality of coffee in Thailand by using a novel yeast strain.</p> sukhumaporn krajangsang, Yaowapa Meeampun, Prapakorn Tantayotai, Titiporn Panyachanakul, Siritron Samosorn, Kulvadee Dolsophon, Rossaporn Jiamjariyatam, Wanlapa Lorliam, Jantima Arnthong, Surisa Suwannarangsee Copyright (c) 2023 Science Essence Journal Fri, 03 Nov 2023 00:00:00 +0000 Cross-docking and molecular dynamic studies to achieve the potent antiviral HIV-1 nonnucleoside reverse transcriptase inhibitors <p>Our objective in the present study is to describe and validate the procedures utilized to investigate a quinoline-based compound's potential as an anti-HIV-1 reverse transcriptase (HIV-1 RT) agent. Through the fusion of the pharmacophores found in the structural makeup of HIV-1 RT drugs, the quinoline derivatives 4-(2′,6′-dimethyl-4′-cyanophenoxy)-6-(4′′-cyanophenyl)-aminoquinoline <strong>(1)</strong> and 4-(2′,6′-dimethyl-4′-cyanophenoxy)-2-(4′′-cyanophenyl)-aminoquinoline <strong>(2)</strong> have been reported and developed. Using cross-docking, molecular docking, and molecular dynamic approaches, the binding interactions of nonnucleoside reverse transcriptase inhibitors (NNRTIs), quinoline derivatives, and HIV-1 RT were examined. When compared to the other conformations of HIV-1 RT, the cross-docking revealed that the 4G1Q.pdb conformation had the lowest binding energy values. According to the molecular docking evidence, <strong>(2)</strong> interacted with LYS101 residues by hydrogen bonding and with TYR181 and TRP229 residues via pi-pi stacking in the binding pocket of HIV-1 RT, similar to that of rilpivirine. Furthermore, molecular dynamics simulations showed that the binding affinity of <strong>(1)</strong> and <strong>(2)</strong> with HIV-1 RT was quite stable. The stronger binding of HIV-1 RT-<strong>(2) </strong>in comparison to HIV-1 RT-<strong>(1) </strong>was further corroborated by the binding free energy determined by MMPBSA and MMGBSA calculations. </p> Suwicha Patnin, Arthit Makarasen, Pongsit Vijitphan, Apisara Baicharoen, Wandee Sirithana, Supanna Techasakul Copyright (c) 2023 Science Essence Journal Fri, 17 Nov 2023 00:00:00 +0000 Influence of Aluminium Contents on the Structure and Hardness of Chromium Aluminium Nitride Thin Films <p>This research aims to study the influence of aluminum content in film on the structure and hardness of chromium aluminum nitride thin films, which are deposited on silicon by reactive DC unbalanced magnetron sputtering method from the Cr-Al alloy target (50:50 at%). The structure, chemical composition, morphology, and hardness were characterized by XRD, EDS, FE-SEM, and nano-indentation techniques, respectively. The results showed that the as-deposited films were formed as a (Cr, Al)N solid solution with fcc structure in the (111), (200), and (220) planes. The as-deposited film had lattice parameters in the range of 4.088-4.138 Å. The as-deposited film had nano-structure whereas the average crystallite size was in the range of 3.1-16.8 nm. The chemical composition from the EDS technique showed that the as-deposited film had chromium (Cr), aluminum (Al), and nitrogen (N) as the main component in different ratios. The FE-SEM micrograph presented that the Al content affected the surface morphology and cross-sectional of the as-deposited film. The hardness, of the as-deposited films measured by the nano-indentation technique, increased from 12.47 GPa to 67.29 GPa with the Al content while decreasing with the crystallite size. The highest hardness found in the CrAlN film with the Al content was 30.93 at% and the crystallite size was 3.1 nm.</p> ADISORN BURANAWONG, Nirun Witit-anun Copyright (c) 2023 Science Essence Journal Fri, 15 Dec 2023 00:00:00 +0000 Effects of Game-Based Learning Management on Learning Achievement in Mathemathics of Geometric Shape and Volume of Rectangular Shape for 6th Grade Students of Srinakharinwirot University Prasarnmit Demonstration School (Elementary) <p>The purpose of this research was to create and determine the efficiency of game-based learning lesson plan on mathematics learning achievement of geometric shape and volume of rectangular shape, to compare learning achievement before and after game-based learning management, and to study satisfaction towards game-based learning management. The samples of the study were 60 students of 6<sup>th</sup> grade at Srinakharinwirot University Prasarnmit Demonstration School (Elementary) in 2<sup>nd</sup> semester of 2022, which was obtained by purposive sampling. The tools used were 5 game-based learning lesson plans for 6<sup>th</sup> grade students (15 periods), a 30-item mathematics learning achievement test of 6<sup>th</sup> grade students and a satisfaction questionnaire on game-based learning management. The research design was the one group pretest-posttest and the statistics used were efficiency determination E1/E2, mean, standard deviation, and t-test for dependent sample. The research results were as follows: 1) The efficiency of process of game-based learning lesson plan which was developed for 6<sup>th</sup> grade students was 87.71 and the efficiency of product was 80.44 which complied to the defined criteria of 80/80; 2) After game-based learning management, 6<sup>th</sup> grade students had higher mathematics learning achievement of geometric shape and volume of rectangular shape than that of before learning at the statistical significance level of .01; 3) After game-based learning management, 6<sup>th</sup> grade students were satisfied with game-based learning management of geometric shape and volume of rectangular shape at the highest level.</p> donsak tsailexthim, Chalothorn Boonthima Copyright (c) 2023 Science Essence Journal Mon, 25 Dec 2023 00:00:00 +0000 Highly Selective and Response NO2 Gas Sensor Based on Au-Coated ZnO Thin Films <p>This paper investigated the nitrogen dioxide and ammonia gas-sensing properties of Au-coated ZnO thin films obtained by a hybrid method combining atomic layer deposition (ALD) and thermal evaporation techniques. The scanning electron microscopy (SEM) results showed Au nanoparticles, and the thickness of the Au-coated ZnO thin films was approximately 33 nm. X-ray photoelectron spectroscopy (XPS) results confirmed that Au nanoparticles were coated on the surface of the ZnO thin films. The gas sensor based on Au-coated ZnO thin films was tested upon exposure to nitrogen dioxide and ammonia gases with concentrations of 10 ppm to 90 ppm at 250 <sup>o</sup>C. Furthermore, the Au-coated ZnO thin films showed an excellent response at a low nitrogen dioxide concentration (10 ppm) with a relative response value of 574%. Moreover, the gas sensor exhibited a high relative and selectivity response to nitrogen dioxide gas.</p> Pitchanunt Chaiyo Copyright (c) 2023 Science Essence Journal Wed, 27 Dec 2023 00:00:00 +0000 Preparation of Polyclonal Antiserum to the Recombinant TiLV-S8 Protein and Its Application in the Detection of Naturally Tilapia Lake Virus (TiLV) Infected Tilapia <p>Tilapia lake virus (TiLV) is classified as a negative-sense, single-stranded RNA virus in the family Amnoonviridae. It is an enveloped virus with 10 genomic RNA segments, each coding for a protein. TiLV causes disease in tilapia, and outbreaks can lead to significant economic losses for the tilapia aquaculture industry. In this study, the gene encoding the segment 8 protein of TiLV was cloned into the expression vector pET15-b and then transformed into <em>Escherichia coli</em> strain BL21. After induction, the recombinant TiLV-S8 protein (rTiLV-S8), with a molecular mass of 20 kDa, was expressed, purified, and used to immunize mice. The mouse antiserum against rTiLV-S8 protein demonstrated specific immunoreactivity for the viral protein, approximately 19 kDa in TiLV-infected fish tissues, as determined by Western blotting. According to the results of the dot blotting assay, the antiserum was about 80 times less sensitive than one-step RT-PCR in detecting TiLV in homogenates of infected fish samples and showed no cross-reaction with uninfected fish tissues, other common fish viruses, or prevalent bacterial species found in aquatic animals. Furthermore, this polyclonal antiserum could be employed to identify TiLV-infected fish in the field using dot blotting assay, and the results can be confirmed by immunohistochemistry.</p> Chatchapong Sopa, Pradit Wangman, Nantawut Ponpukdee, Parin Chaivisuthangkura, Siwaporn Longyant Copyright (c) 2023 Science Essence Journal Wed, 27 Dec 2023 00:00:00 +0000